ABSTRACT
This paper firstly analyzed from three aspects:the necessity of pharmacognosy medical ethics educa-tion, and then illustrates the main content of the medicine ethics education , this paper expounds the method and form of medicine ethics education , and finally summarizes the medicine ethics education for clinical practice stage of medical students experience:the medical institutions about the medicine ethics instances of intuitive for training medical students , strengthening medical students learn the importance of pharmaceutical ethics in clinical practice stage , in order to enhance the medical students medical moral sentiment , lay the foundation for clinical drug safety work.
ABSTRACT
OBJECTIVE:To improve the HPLC-fluorometric method in the determination of telmisartan in human plasma.METHODS:Using naproxen as internal standard,the plasma was extracted with ethyl acetate.The separation was performed on Symmetry C8 with mobile phase consisted of acetonitrile -0.01mol?L-1 potassium dihydrogen phosphate buffer solution(47∶53) at a flow rate of 1.0ml?min-1.The ?Ex was set at 305nm and the ?Em was set at 365nm,and the sample size was 20?L.RESULTS:The linear range of telmisartan was 2.5～200ng?mL-1(r=0.999 8),with the lowest detectable limit at 1ng?mL-1.The average recovery of extraction was (89.91?10.07)%.Both the intra-day RSD and inter-day RSD were less than 10%.The sample showed a good stability within 24 hours after three freeze-thaw cycles and extractions.CONCLUSI-ON:The method is simple,sensitive,accurate and reproducible,and suitable for the determination and pharmacokinetic study of telmisartan in human plasma.